PHYTO-PAM instruments facilitate measurements of mixed algae populations for analysis of composition and differences in the photosynthetic performance of up to four algae groups within one sample. These four phytoplankton groups have antennae with different spectral properties. Deconvolution is based on differences in the fluorescence emission induced by multiple wavelength excitation. The instruments feature extremely sensitive fluorescence detection down to a concentration of 0.1 µg Chl a/l; concentrations that are typically found in natural surface waters or coastal seawater. Differential analysis of the sample by PAM measurements such as saturation pulse quenching analysis, determination of effective PS II quantum yield, light response curves, and induction curves provide comprehensive data on the photosynthetic performance of the diverse algae. Additionally, a fast kinetics mode for assessment of the wavelength-dependent absorption cross section of PS II, σII(λ), based on the evaluation of the O-I1 kinetics is provided for single cultures.
Reference Exchange Database for PHYTO-PAM-II
The classification of algal groups is fitted with reference spectra specifying the F-excitation properties of the respective algae. PHYTO-PAM-II instruments feature universal interchangeable reference files. Here we provide a reference exchange database.
You are welcome to upload / download PHYTO-PAM-II reference files. Content and quality of the references have not been validated by Heinz Walz GmbH.
PHYTO-PAM-II chlorophyll fluorometers feature five measuring light wavelengths (440, 480, 540, 590 and 625 nm) for simultaneous excitation and deconvolution of four algae groups. The same five colors plus white are provided for actinic illumination and determination of wavelength-dependent absorption cross-section.
PHYTO-PAM-II is currently available in two versions: a COMPACT version and a MODULAR version. In addition, a FIBER version is available with four excitation wavelengths (470, 520, 645 and 665 nm) and one actinic light source (655 nm).