Multiple Excitation Wavelength Chlorophyll Fluorescence Analyzer
The MULTI-COLOR-PAM fluorometer provides an unprecedented color range of measuring and actinic light including white and far-red light sources. The various colors enable researchers to tailor conditions of fluorescence measurements exactly to the spectral properties of their sample.
Therefore, the MULTI-COLOR-PAM fluorometer opens the way to study many wavelength-dependent aspects of photosynthesis that so-far have not been commonly accessible.
General Features MULTI-COLOR-PAM
The MULTI-COLOR-PAM provides 6 colors of pulse-modulated measuring light (400, 440, 480, 540, 590 and 625 nm) and 5 colors of actinic light (440, 480, 540, 590, 625). In addition, white (420-640 nm) and far-red light (730 nm) light sources are built in.
By employing state-of-the-art LED light sources in combination with elaborated chip-on-board technology, strong actinic photon fluxes are achieved while keeping the emitter unit highly compact. Each measuring light color can be freely combined with one of the available actinic light sources.
The MULTI-COLOR-PAM detects fluorescence by a highly sensitive photodiode attached to either an optical unit for suspensions or for leaves. A spherical or flat cosine-corrected sensor feeds its signal directly to the MULTI-COLOR-PAM control unit so that accurate PAR measurements can be obtained in suspension or at leaf surfaces. A special routine is provided to measure PAR-lists for all colors automatically.
The MULTI-COLOR-PAM detects effective PS II quantum yield, Y(II), with outstanding accuracy and, thus, enables highly reliable calculations of relative electron transport rates (rel. ETRmax) even with extremely stressed samples.
Outstanding features of the MULTI-COLOR-PAM
- The outstanding feature of this instrument is its versatility.
- Six measuring light colors, five actinic light colors plus white light.
- Combining outstanding sensitivity with high time resolution (10 µs).
- Ideal for fast kinetic measurements of reaction center closure and opening, and carotenoid radicals (HIQ).
- Special configuration available to measure epidermal UV-A screening.
The MULTI-COLOR-PAM fluorometer is optimized for highly sensitive assessment of photosynthesis in dilute suspensions of algae, cyanobacteria and chloroplasts. In addition, the measuring system can be configured to study leaf photosynthesis and epidermal UV-A screening.
Data analysis by the MULTI-COLOR-PAM fluorometer includes the option to determine the wavelength-dependent functional PS II absorption cross-section, Sigma(II)λ, and absolute PS II turnover rates, ETR(II)λ. In addition, the MULTI-COLOR-PAM software provides standard saturation pulse quenching analysis and methods for analysis of fast fluorescence changes including polyphasic rise kinetics and dark relaxation of fluorescence with a time resolution of 10 μs.
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